Assays can be better optimized by scanning across. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. The drawing shows a standard microplate of 15 mm height covered with a lid. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. . In addition, the SpectraMax® iD5 can be expanded to include bottom. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. SpectraMax Mini User Guide. 2. Step 4: Incubate at room temperature for 1 hour, protected from light. keeping with best practices, reader performance should. 5 12. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The SpectraMax® iD3 Multi Mode Microplate . The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Omnia® Compatible Microplate Reader Documentation Version No. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. 6% for the on-board optics (monochromators). The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. AlphaScreen. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Microplate reader settings. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. . 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. Compact designs. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The calculated LOQ values were 0. 0 out of 5 $ 23,995 $ 22,495. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. flexibility of monochromator-based systems. The advanced optical and. The system uses two scanning monochromators to determine the optimal excitation and emission settings. 5 12. Examples: “Press carefully to engage,” “ Do not press down. 315 can't find zero order. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. These lab workhorses are used in drug discovery, research, bioassay. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Thank you!. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 7°C and the bottom to 36. Sale. We would like to show you a description here but the site won’t allow us. Cuvette Microplate wells. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. Intended Use/Discipline:Biological. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Figure 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . Results are shown in Table 1. For readers without a cuvette port, simply check the box next to ‘PathCheck’. 1. begin reading microplates with seven mouse clicks. 0 out of 5 $ 5,999. The system uses two scanning monochromators to determine the optimal excitation and emission settings. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. ELISA Plate Readers / Microplate Readers. Figure 1. 313 reference gain check fail. SpectraMax i3x Multi-Mode Detection Platform. The powerful analysis tools in the software generate a standard curve and calculated data. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. When the user applies a set temperature of 37°C, the top is heated to 37. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. View the Molecular Devices website. Add to Cart. SoftMax® Pro 7. The plate reader can measure multiple readouts, including UV. Italic and boldface type are used for emphasis. Contact our support team with your questions regarding Molecular Devices products and applications. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 100 fmol 1536-well (8 µL) 0. The following document is intended to demonstrate setup of this instrument. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Table 2 summarizes the results for the uniformity test. In addition, the SpectraMax iD5 can be expanded to. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Download the SpectraMax Mini Reader Plug-in and associated guides. Fully tested with validations, warranty. 306 plate air cal. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. 7°C (gradient heating design). This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. With optimized reagents, option to operate the. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Molecular Devices Spectramax iD3 Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The 2-channel configuration is available with 0, 2, or 4 injectors. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. 1. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. , April 14, 2022 – Molecular Devices, LLC. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Three low volume 24-well bottom slides. 1. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. Tecan Infinite Microplate Reader. Add to Cart. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 1 software Download page. Assays vary depending on. Example: [Drawer]. Page 28 Chapter 5: Read Modes and Read Types 1. Our specialization servicing. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. 303 unable to cal dark current. . When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. no. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. If it looks fine, then proceed. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Step 2: Launch SoftMax Pro Software. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Blank subtraction and standard curve fitting were done automatically by the software. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Products : General, Microplate Readers. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. A preconfigured protocol is available in the software to. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 380 nm/Exc. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. SpectraMax Spectrofluorometers. Here, the CVs for each plate were less than 3, and the difference between mean. It measures the optical density (OD) of samples in both 96- and 384-well. This unlocks the transport slide. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. flat meniscus), but is approx 15 mL. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. These robust readers have been placed in labs from. 76358-624. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Other technologies such as imaging. We would like to show you a description here but the site won’t allow us. Also see:. The calculated LOQ values were 0. On the back, in the right hand top corner,. Detailed specifications of each model reader are reported in Section. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Temperature control. The dual monochromator optics allow. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. See SpectraMax® Microplate Reader User Manual Download Page. . Microplate Readers BioTek Epoch 2 Microplate Reader. 2. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. 2 only) SpectraMax M-series Microplate readers. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Open the top cover of the instrument. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Molecular Devices. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Selection of Water Constant in PathCheck settings. When the user applies a set temperature of 37°C, the top is heated to 37. Examples: “Press carefully to engage,” “ Do not. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Italic and boldface type are used for emphasis. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. Selection of Water Constant in PathCheck settings. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. These lab workhorses are used in drug discovery. 2. . The Gemini EM can sample a variety of formats, from 6- to 384-well. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Read More. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Turn the reader off by pulling the power cord. 1) Select the correct plate and the wells that you want to measure. 0 out of 5 $ 9,995 $ 9,495. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. microplate reader. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. For a higher . Molecular Devices SpectraMax M2E Microplate Reader. Next generation SpectraMax® absorbance microplate readers. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. To resume collecting kinetic measurements within the same file, press the Append button. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. 1. Category 2 and 3 microplate readers use gradient heating as well. Step 1: Turn on your microplate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 305 reference level saturation. Cell Analysis. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. Add to Cart. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. both cuvette-based and microplate reader applications on the same instrument. Published Dec 16, 2020 | Updated Dec 16, 2020. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. Sensitive fluorescent quantitation of DNA with the Quant-iT. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Use the provided 3. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Devices’ StakMax® Microplate Stacker in a matter of minutes and . their consistent performance, reliability and durability. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. Align the two slots on the bottle holder with the two pegs on the rail. SpectraMax® Microplate Readers and SoftMax® Pro Software. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Microplate automation and detection instruments for walk away operation and high quality results. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. 2 software: see SoftMax® Pro 7. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Maximum signal with lowest background. Other features and benefits include: Eliminate changing out filters. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The cuvette must be in place when you read the plate. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. Measuring platelet aggregation with a microplate reader. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Please refer to the Obsolete Microplate Reader Models for more information. . The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 313 reference gain check fail. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. SpectraDrop High-Throughput Screening (HTS) Kit. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The advanced optical and. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. Multimode Microplate Reader. Note that this procedure is only for the SpectraMax L model. 7°C and the bottom to 36. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. edu) to be added as a trained user in iLab. In addition, the iD5 reader measures TRF and FP. 0200-6263. In . Nucleic Acid and Protein Quantitation. 300 µL of Detergent Compatible Bradford reagent was added to each well. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. . . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Avoid losing high signal. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Click SoftMax Pro Software User Guide. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. For readers without a cuvette port, these options are not shown in the software. Unlock your i3/i3x instrument after shipment. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Access this by going to Help >. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Wide range of applications. The CellTiter-Glo assay from Promega uses luciferase. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The price range of a reader starts at 2. It can run samples based on pre-defined. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein.